604 research outputs found
Agricultura orgĂąnica em ĂĄreas urbanas e periurbanas com base na agroecologia.
A agricultura orgĂąnica com base na agroecologia Ă© o mote tecnolĂłgico adequado Ă realidade dos agroecossistemas urbanos. Este artigo ressalta a necessidade de se desenvolver tecnologias e insumos especĂficos. A partir de experiĂȘncias com agricultura urbana em diferentes paĂses em desenvolvimento, evidencia-se a necessidade de se buscar capacidades locais e apoio do poder pĂșblico, especialmente nas iniciativas da sociedade organizada e mobilizada para a produção agrĂcola urbana
Proteomics-based identification of differentially abundant proteins reveals adaptation mechanisms of Xanthomonas citri subsp citri during Citrus sinensis infection
Background: Xanthomonas citri subsp. citri (Xac) is the causal agent of citrus canker. A proteomic analysis under in planta infectious and non-infectious conditions was conducted in order to increase our knowledge about the adaptive process of Xac during infection. Results: For that, a 2D-based proteomic analysis of Xac at 1, 3 and 5 days after inoculation, in comparison to Xac growth in NB media was carried out and followed by MALDI-TOF-TOF identification of 124 unique differentially abundant proteins. Among them, 79 correspond to up-regulated proteins in at least one of the three stages of infection. Our results indicate an important role of proteins related to biofilm synthesis, lipopolysaccharides biosynthesis, and iron uptake and metabolism as possible modulators of plant innate immunity, and revealed an intricate network of proteins involved in reactive oxygen species adaptation during Plants'Oxidative Burst response. We also identified proteins previously unknown to be involved in Xac-Citrus interaction, including the hypothetical protein XAC3981. A mutant strain for this gene has proved to be non-pathogenic in respect to classical symptoms of citrus canker induced in compatible plants. Conclusions: This is the first time that a protein repertoire is shown to be active and working in an integrated manner during the infection process in a compatible host, pointing to an elaborate mechanism for adaptation of Xac once inside the plant.Fundacao de Amparo a Pesquisa do Estado de Sao PauloFundacao de Amparo a Pesquisa do Estado de Minas GeraisBIGA grant-CAPESUniv Fed Ouro Preto, Inst Ciencias Exatas & Biol, Dept Ciencias Biol DECBI, Ouro Preto, MG, BrazilUniv Fed Ouro Preto, Nucleo Pesquisas Ciencias Biol NUPEB, Ouro Preto, MG, BrazilUniv Fed Rio de Janeiro, Inst Quim, Dept Bioquim DBq, Rio De Janeiro, RJ, BrazilUniv Estadual Paulista, UNESP, Dept Tecnol, Fac Ciencias Agr & Vet Jaboticabal, Jaboticabal, SP, BrazilUniv Estadual Campinas, UNICAMP, Inst Quim, Campinas, SP, BrazilUniv Sao Paulo, Inst Quim, Dept Bioquim, Sao Paulo, SP, BrazilUniv Fed Sao Paulo UNIFESP, Dept Ciencias Biol, Diadema, SP, BrazilVirginia Tech, Biocomplex Inst, Blacksburg, VA USAUniv Sao Paulo, Inst Quim, Dept Bioquim, Sao Paulo, SP, BrazilUniv Fed Sao Paulo UNIFESP, Dept Ciencias Biol, Diadema, SP, BrazilCAPESFAPESP: 04/02006-7Fundacao de Amparo a Pesquisa do Estado de Minas Gerais: CBB-APQ-04425-10BIGA grant-CAPES: 3385/2013Web of Scienc
Detection and identification of Xanthomonas pathotypes associated with citrus diseases using comparative genomics and multiplex PCR
Background. In Citrus cultures, three species of Xanthomonas are known to cause distinct diseases. X. citri subsp. citri patothype A, X. fuscans subsp. aurantifolii pathotypes B and C, and X. alfalfae subsp. citrumelonis, are the causative agents of cancrosis A, B, C, and citrus bacterial spots, respectively. Although these species exhibit different levels of virulence and aggressiveness, only limited alternatives are currently available for proper and early detection of these diseases in the fields. The present study aimed to develop a new molecular diagnostic method based on genomic sequences derived from the four species of Xanthomonas. Results. Using comparative genomics approaches, primers were synthesized for the identification of the four causative agents of citrus diseases. These primers were validated for their specificity to their target DNA by both conventional and multiplex PCR. Upon evaluation, their sensitivity was found to be 0.02 ng/mu l in vitro and 1.5 x 10(4) CFU ml(-1) in infected leaves. Additionally, none of the primers were able to generate amplicons in 19 other genomes of Xanthomonas not associated with Citrus and one species of Xylella, the causal agent of citrus variegated chlorosis (CVC). This denotes strong specificity of the primers for the different species of Xanthomonas investigated in this study. Conclusions. We demonstrated that these markers can be used as potential candidates for performing in vivo molecular diagnosis exclusively for citrus-associated Xanthomonas. The bioinformatics pipeline developed in this study to design specific genomic regions is capable of generating specific primers. It is freely available and can be utilized for any other model organism.7CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTĂFICO E TECNOLĂGICO - CNPQCOORDENAĂĂO DE APERFEIĂOAMENTO DE PESSOAL DE NĂVEL SUPERIOR - CAPESFUNDAĂĂO DE AMPARO Ă PESQUISA DO ESTADO DE MINAS GERAIS - FAPEMIG481226/2013-3CFP 51/2013; 3385/2013APQ-02387-1
Status and Recent Results of the Acoustic Neutrino Detection Test System AMADEUS
The AMADEUS system is an integral part of the ANTARES neutrino telescope in
the Mediterranean Sea. The project aims at the investigation of techniques for
acoustic neutrino detection in the deep sea. Installed at a depth of more than
2000m, the acoustic sensors of AMADEUS are based on piezo-ceramics elements for
the broad-band recording of signals with frequencies ranging up to 125kHz.
AMADEUS was completed in May 2008 and comprises six "acoustic clusters", each
one holding six acoustic sensors that are arranged at distances of roughly 1m
from each other. The clusters are installed with inter-spacings ranging from
15m to 340m. Acoustic data are continuously acquired and processed at a
computer cluster where online filter algorithms are applied to select a
high-purity sample of neutrino-like signals. 1.6 TB of data were recorded in
2008 and 3.2 TB in 2009. In order to assess the background of neutrino-like
signals in the deep sea, the characteristics of ambient noise and transient
signals have been investigated. In this article, the AMADEUS system will be
described and recent results will be presented.Comment: 7 pages, 8 figures. Proceedings of ARENA 2010, the 4th International
Workshop on Acoustic and Radio EeV Neutrino Detection Activitie
Study of ZÎł events and limits on anomalous ZZÎł and Zγγ couplings in ppÌ collisions at s=1.96TeV
We present a measurement of the ZÎł production cross section and limits on anomalous ZZÎł and Zγγ couplings for form-factor scales of Î=750 and 1000 GeV. The measurement is based on 138 (152) candidates in the eeÎł (ÎŒÎŒÎł) final state using 320(290)pb-1 of ppÌ collisions at s=1.96TeV. The 95% C.L. limits on real and imaginary parts of individual anomalous couplings are |h10,30Z|<0.23, |h20,40Z|<0.020, |h10,30Îł|<0.23, and |h20,40Îł|<0.019 for Î=1000GeV. © 2005 The American Physical Society
The ANTARES Telescope Neutrino Alert System
The ANTARES telescope has the capability to detect neutrinos produced in
astrophysical transient sources. Potential sources include gamma-ray bursts,
core collapse supernovae, and flaring active galactic nuclei. To enhance the
sensitivity of ANTARES to such sources, a new detection method based on
coincident observations of neutrinos and optical signals has been developed. A
fast online muon track reconstruction is used to trigger a network of small
automatic optical telescopes. Such alerts are generated for special events,
such as two or more neutrinos, coincident in time and direction, or single
neutrinos of very high energy.Comment: 17 pages, 9 figures submitted to Astroparticle Physic
Acoustic and optical variations during rapid downward motion episodes in the deep north-western Mediterranean Sea
An Acoustic Doppler Current Profiler (ADCP) was moored at the deep-sea site
of the ANTARES neutrino telescope near Toulon, France, thus providing a unique
opportunity to compare high-resolution acoustic and optical observations
between 70 and 170 m above the sea bed at 2475 m. The ADCP measured downward
vertical currents of magnitudes up to 0.03 m s-1 in late winter and early
spring 2006. In the same period, observations were made of enhanced levels of
acoustic reflection, interpreted as suspended particles including zooplankton,
by a factor of about 10 and of horizontal currents reaching 0.35 m s-1. These
observations coincided with high light levels detected by the telescope,
interpreted as increased bioluminescence. During winter 2006 deep dense-water
formation occurred in the Ligurian subbasin, thus providing a possible
explanation for these observations. However, the 10-20 days quasi-periodic
episodes of high levels of acoustic reflection, light and large vertical
currents continuing into the summer are not direct evidence of this process. It
is hypothesized that the main process allowing for suspended material to be
moved vertically later in the year is local advection, linked with topographic
boundary current instabilities along the rim of the 'Northern Current'.Comment: 30 pages, 7 figure
Search for right-handed W bosons in top quark decay
We present a measurement of the fraction f+ of right-handed W bosons produced
in top quark decays, based on a candidate sample of events in the
lepton+jets decay mode. These data correspond to an integrated luminosity of
230pb^-1, collected by the DO detector at the Fermilab Tevatron
Collider at sqrt(s)=1.96 TeV. We use a constrained fit to reconstruct the
kinematics of the and decay products, which allows for the
measurement of the leptonic decay angle for each event. By comparing
the distribution from the data with those for the expected
background and signal for various values of f+, we find
f+=0.00+-0.13(stat)+-0.07(syst). This measurement is consistent with the
standard model prediction of f+=3.6x10^-4.Comment: Submitted to Physical Review D Rapid Communications 7 pages, 3
figure
Measurement of Semileptonic Branching Fractions of B Mesons to Narrow D** States
Using the data accumulated in 2002-2004 with the DO detector in
proton-antiproton collisions at the Fermilab Tevatron collider with
centre-of-mass energy 1.96 TeV, the branching fractions of the decays B ->
\bar{D}_1^0(2420) \mu^+ \nu_\mu X and B -> \bar{D}_2^{*0}(2460) \mu^+ \nu_\mu X
and their ratio have been measured: BR(\bar{b}->B) \cdot BR(B-> \bar{D}_1^0
\mu^+ \nu_\mu X) \cdot BR(\bar{D}_1^0 -> D*- pi+) =
(0.087+-0.007(stat)+-0.014(syst))%; BR(\bar{b}->B)\cdot BR(B->D_2^{*0} \mu^+
\nu_\mu X) \cdot BR(\bar{D}_2^{*0} -> D*- \pi^+) =
(0.035+-0.007(stat)+-0.008(syst))%; and (BR(B -> \bar{D}_2^{*0} \mu^+ \nu_\mu
X)BR(D2*0->D*- pi+)) / (BR(B -> \bar{D}_1^{0} \mu^+ \nu_\mu X)\cdot
BR(\bar{D}_1^{0}->D*- \pi^+)) = 0.39+-0.09(stat)+-0.12(syst), where the charge
conjugated states are always implied.Comment: submitted to Phys. Rev. Let
Search for Large Extra Spatial Dimensions in Dimuon Production with the D0 Detector
We present the results of a search for the effects of large extra spatial
dimensions in collisions at 1.96 TeV in events
containing a pair of energetic muons. The data correspond to 246 \ipb of
integrated luminosity collected by the \D0 experiment at the Fermilab Tevatron
Collider. Good agreement with the expected background was found, yielding no
evidence for large extra dimensions. We set 95% C.L. lower limits on the
fundamental Planck scale between 0.85 TeV and 1.27 TeV within several
formalisms. These are the most stringent limits achieved in the dimuon channel
to date.Comment: 8 pages, 3 figures, 1 table. Published in Phys. Rev. Lett. Minor
changes in v2 to match the published versio
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